Primers for wheat SNP-Chips
The following files contain designed primers to known markers
Wheat Axiom 820k
The Axiom chip was developed by the WISP, for further information go here
- Located against Chinese Spring RefSeq 1.0. The markers where extracted from ensembl plants, release 40.
The primers where designed against the pseudomolecules described in IWGSC(2018).
Wheat iSelect 90k
The markers in this section are from the Illumina iSelect chip described in Wang (2014).
- Located against CSS 81,587 SNPs located according to the best hit to the scaffolds from the IWGSC
- Genetically mapped 46,977 SNPs located according to the genetic map.
The primers were designed against the soft-masked reference as released in ensembl plants IWGSC1. For an updated version of the primers, please look at the primers of the Wheat Axiom 820k section above, which contains the SNPs in the iSelect chip.
: International Wheat Genome Sequencing Consortium (2018), Shifting the limits in wheat research and breeding using a fully annotated reference genome. Science, 361(eaar7191). doi: 10.1126/science.aar7191
: Wang, S., Wong, D., Forrest, K., et al. (2014), Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array. Plant Biotechnology Journal, 12: 787-796. doi:10.1111/pbi.12183
: International Wheat Genome Sequencing Consortium. (2014). A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome. Science, 345(6194), 1251788-1251788. doi:10.1126/science.1251788